Composite

Part:BBa_K1462400:Design

Designed by: Ye Peng & Junjie Zhang   Group: iGEM14_SCUT   (2014-10-10)


pGAL1+PRK+GBD-ligand+ADH1+pTDH3+RuBisCo+SH3-ligand+ADH1+pTDH3+CA+PDZ-ligand+ADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 649
    Illegal BglII site found at 4953
    Illegal BglII site found at 5979
    Illegal BamHI site found at 6797
    Illegal XhoI site found at 6778
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the complete molecule for pathway engineering of outer mitochondrial membrane.



Source

Kits and a synthesis company.

References